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Stigma/style cysteine-rich adhesin (SCA), a lily LTP, is secreted from both pollen and the pistil transmitting tract epidermis (TTE), where pollen tubes grow and are guided to the ovules (Lord, 2000).
For examination of gene expression in response to abiotic stresses, all seedlings were germinated on sterilized filter paper soaked with liquid MS under normal growth conditions for 2 d. 3B, arrowheads, C) or a single plant having two inflorescences (Fig. The values on the branches indicate the number of bootstrap replicates supporting the branch.
Young seedlings were then moved to new MS plates with each stress condition and grown further for 3 d. Only bootstrap replication values Phylogenetic relationships of plant LTP and LTP-like proteins.
The heuristic search found 232 equally most parsimonious trees (tree length=7584, consistency index=0.4248, retention index=0.5050), and tree topologies were very similar to the NJ tree.
Therefore, the discussion was based on the strict consensus tree of MP analysis.
SCAs can bind the pectin matrix until 50 m M Na Cl is added (Fig. The low p H can facilitate activity of an ECM protein such as expansin (Cosgrove, 2005).
In a previous study of SCA proteins (Chae , 2007), the calculated titration curves of SCAs showed plateau regions of about the same net charge in the p H range of 4 to ∼7.
grid with the same dimensions for all protein structures.
After superimposition of their three-dimensional coordinates, the structures were centred in an identical way in the grid.
GUS-stained tissues were decolourized with 70% Et OH three times in a 12 h incubation at 37 °C. Plants were grown and wild-type plants grew ∼2 cm tall and 3–4 cm tall per day, respectively. 3G) and supernumerary branches at a single node (Fig. Each type of tissue fusion occurred randomly in Phylogenetic relationships of plant LTP and LTP-like proteins.
Developed GUS signals were examined under a Leica dissecting microscope. (B) Two leaves (arrowheads) congenitally fused onto a single petiole. (D) Two inflorescences (arrows) fused into a single primary axis. (F) Two primary stems (arrows) emerging at the bolting stage. The difference in stature at day 5 (∼10 cm) was maintained until day 12 at plant maturity (stage 6.5, 50% flowers opened) (Boyes =50) showed abnormal organ fusions such as two leaves congenitally fused onto a single petiole (Fig. Some showed two primary stems separately emerging from the shoot apical meristem at the bolting stage (Fig. This tree represents a strict consensus tree of 232 equally most parsimonious trees based on maximum parsimony analysis.